Read the following statements.

(A) Today more than 900 restriction enzymes that has been isolated from over 230 strains of bacteria.

(B) Restriction enzyme belongs to larger class of enzymes called nucleases. These are of two kind; exonucleases and endonucleases.

(C) Exonuclease enzymes remove nucleotide from ends of DNA whereas endonuclease make cut at specific points within DNA.

(D) Restriction endonuclease cut each of two DNA strands of double helix at specific points in their sugar-phosphate backbone.

(E) Each restriction enzyme recognises specific palindromic nucleotide sequence in DNA.

(F) When restriction enzymes cut the strand of DNA away from centre of palindrome sites, it produces overhanging stretches known as sticky ends.

(G) The DNA fragments can be separated by technique known as gel electrophoresis based on size.

(H) DNA fragments being negatively charged move towards cathode under electric field through matrix in gel electrophoresis.

(I) The most common used matrix in gel electrophoresis is agarose, which is synthetic polymer extracted from sea weeds.

(J) The separated DNA fragments in gel electrophoresis can be visualised after staining the DNA with compound ethidium bromide followed by exposure to UV radiation. It is seen as orange coloured band.

(K) The process wherein the separated bands of DNA in gel electrophoresis are cut out from agarose gel and extracted from the gel piece is called sieving effect.

Choose the option with all incorrect statements :