What is true about packaging of DNA Helix in eukaryotes?

(i) Negatively charged DNA is wrapped around the positively charged basic histone proteins octamer to form a structure called nucleosome.

(ii) A typical nucleosome contains 200 bp of DNA helix.

(iii) Nucleosomes constitute the repeating unit of a structure in nucleus called chromatin.

(iv) The nucleosomes in chromatin are seen as 'beads-on-string' structure when viewed under electron microscope (EM).

(v) Chromatin fibres are further coiled and condensed at metaphase stage of cell division to form chromosomes.

(vi) The packaging of chromatin at higher level requires additional set of proteins that collectively are referred to as Non-Histone Chromosomal (NHC) proteins.